標題: Candida albicans ENO1 null mutants exhibit altered drug susceptibility, hyphal formation, and virulence
作者: Ko, Hui-Ching
Hsiao, Ting-Yin
Chen, Chiung-Tong
Yang, Yun-Liang
生物科技學系
分子醫學與生物工程研究所
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
關鍵字: enolase;opportunistic human pathogen;drug susceptibility;hyphal formation;virulence
公開日期: 1-六月-2013
摘要: We previously showed that the expression of ENO1 (enolase) in the fungal pathogen Candida albicans is critical for cell growth. In this study, we investigate the contribution of the ENO1 gene to virulence. We conducted our functional study of ENO1 in C. albicans by constructing an eno1/eno1 null mutant strain in which both ENO1 alleles in the genome were knockouted with the SAT1 flipper cassette that contains the nourseothricin-resistance marker. Although the null mutant failed to grow on synthetic media containing glucose, it was capable of growth on media containing yeast extract, peptone, and non-fermentable carbon sources. The null mutant was more susceptible to certain antifungal drugs. It also exhibited defective hyphal formation, and was avirulent in BALB/c mice.
URI: http://dx.doi.org/10.1007/s12275-013-2577-z
http://hdl.handle.net/11536/22318
ISSN: 1225-8873
DOI: 10.1007/s12275-013-2577-z
期刊: JOURNAL OF MICROBIOLOGY
Volume: 51
Issue: 3
起始頁: 345
結束頁: 351
顯示於類別:期刊論文


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