標題: Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy
作者: Li, Hsing-Yuan
Tseng, Shing-Hua
Cheng, Tsai-Mu
Chu, Hsueh-Liang
Lu, Yu-Ning
Wang, Fang-Yu
Tsai, Li-Yun
Shieh, Juo-Yu
Yang, Jyh-Yuan
Juan, Chien-Chang
Tu, Lung-Chen
Chang, Chia-Ching
生物科技學系
Department of Biological Science and Technology
公開日期: 19-七月-2013
摘要: Enterovirus 71 (EV71) infection is an emerging infectious disease causing neurological complications and/or death within two to three days after the development of fever and rash. A low viral titre in clinical specimens makes the detection of EV71 difficult. Conventional approaches for detecting EV71 are time consuming, poorly sensitive, or complicated, and cannot be used effectively for clinical diagnosis. Furthermore, EV71 and Coxsackie virus A16 (CA16) may cross react in conventional assays. Therefore, a rapid, highly sensitive, specific, and user-friendly test is needed. We developed an EV71-specific nanogold-modified working electrode for electrochemical impedance spectroscopy in the detection of EV71. Our results show that EV71 can be distinguished from CA16, Herpes simplex virus, and lysozyme, with the modified nanogold electrode being able to detect EV71 in concentrations as low as 1 copy number/50 mu 1 reaction volume, and the duration between sample preparation and detection being 11 min. This detection platform may have the potential for use in point-of-care diagnostics.
URI: http://dx.doi.org/10.1088/0957-4484/24/28/285102
http://hdl.handle.net/11536/22191
ISSN: 0957-4484
DOI: 10.1088/0957-4484/24/28/285102
期刊: NANOTECHNOLOGY
Volume: 24
Issue: 28
結束頁: 
顯示於類別:期刊論文


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