標題: PAComplex: a web server to infer peptide antigen families and binding models from TCR-pMHC complexes
作者: Liu, I-Hsin
Lo, Yu-Shu
Yang, Jinn-Moon
生物科技學系
生物資訊及系統生物研究所
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
公開日期: 1-七月-2011
摘要: One of the most adaptive immune responses is triggered by specific T-cell receptors (TCR) binding to peptide-major histocompatibility complexes (pMHC). Despite the availability of many prediction servers to identify peptides binding to MHC, these servers are often lacking in peptide-TCR interactions and detailed atomic interacting models. PAComplex is the first web server investigating both pMHC and peptide-TCR interfaces to infer peptide antigens and homologous peptide antigens of a query. This server first identifies significantly similar TCR-pMHC templates (joint Z-value >= 4.0) of the query by using antibody-antigen and protein-protein interacting scoring matrices for peptide-TCR and pMHC interfaces, respectively. PAComplex then identifies the homologous peptide antigens of these hit templates from complete pathogen genome databases (>= 10(8) peptide candidates from 864 628 protein sequences of 389 pathogens) and experimental peptide databases (80 057 peptides in 2287 species). Finally, the server outputs peptide antigens and homologous peptide antigens of the query and displays detailed interacting models (e.g. hydrogen bonds and steric interactions in two interfaces) of hitTCR-pMHC templates. Experimental results demonstrate that the proposed server can achieve high prediction accuracy and offer potential peptide antigens across pathogens. We believe that the server is able to provide valuable insights for the peptide vaccine and MHC restriction. The PAComplex sever is available at http://PAcomplex.life.nctu.edu.tw.
URI: http://dx.doi.org/10.1093/nar/gkr434
http://hdl.handle.net/11536/22076
ISSN: 0305-1048
DOI: 10.1093/nar/gkr434
期刊: NUCLEIC ACIDS RESEARCH
Volume: 39
Issue: 
起始頁: W254
結束頁: W260
顯示於類別:期刊論文


文件中的檔案:

  1. 000292325300042.pdf