標題: Evidence of securin-mediated resistance to gefitinib-induced apoptosis in human cancer cells
作者: Yu, Sheng-Yi
Liu, Huei-Fang
Wang, Su-Pei
Chang, Chia-Ching
Tsai, Chuan-Mei
Chao, Jui-I
生物科技學系
分子醫學與生物工程研究所
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
關鍵字: Gefitinib;Securin;Apoptosis;EGFR;Cancer cells
公開日期: 25-Apr-2013
摘要: Gefitinib, a tyrosine kinase inhibitor of the epidermal growth factor receptor (EGFR), has been used to treat numerous cancers; however, evidence has shown that cancer cells can become resistant to gefitinib during therapy. Here, we report a human proto-oncogene, securin, which displays resistance to death in cancer cells. Gefitinib treatment decreases securin levels at the protein level by inducing protein instability but did not affect on the securin gene expression. Treatment with gefitinib induced cytotoxicity in various human cancer cell types, including RKO (colon cancer), A549 (lung cancer), BFTC905 (bladder cancer), MCF7 (breast cancer) and A375 (skin cancer). BFTC905 and A549 cells expressed relatively high levels of the phosphorylated and total EGFR proteins; however, A375, MCF7 and RKO cells did not markedly express these proteins. Moreover, following treatment with gefitinib, the securin-wild type cancer cells were more resistant to apoptotic induction than the securin-null cancer cells. Surprisingly, both the securin-wild type and securin-null cancer cells expressed the EGFR protein at similar levels. Treatment with gefitinib induced mitochondrial dysfunction, cytochrome c release, caspase-3 activation and poly (ADP-ribose) polymerase protein cleavage, indicating that apoptosis occurred in these cancer cells. The transfection of a GPF-securin expression vector increased both the proliferation rates and resistance to gefitinib-induced death in these cancer cells. Taken together, these findings demonstrate that the presence of securin promotes resistance to gefitinib-induced apoptosis via an EGFR-independent pathway in human cancer cells. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.cbi.2013.03.011
http://hdl.handle.net/11536/21924
ISSN: 0009-2797
DOI: 10.1016/j.cbi.2013.03.011
期刊: CHEMICO-BIOLOGICAL INTERACTIONS
Volume: 203
Issue: 2
起始頁: 412
結束頁: 422
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