標題: In vivo magnetic resonance imaging of mice liver tumors using a new gadolinium-based contrast agent
作者: Chen, Shih-Hsien
Kuo, Yu-Ting
Cheng, Tian-Lu
Chen, Chiao-Yun
Chiu, Yen-Yu
Lai, Jui-Jen
Chang, Chih-Ching
Jaw, Twei-Shiun
Wang, Yun-Ming
Liu, Gin-Chung
生物科技學系
Department of Biological Science and Technology
關鍵字: Contrast;beta-glucuronidase;Mouse liver;MRI
公開日期: 1-May-2013
摘要: We compared the enhancement effect between a newly synthesized tissue-specific contrast agent, [Gd-DOTA-FP beta G], and a commercially available agent, [Gd(DOTA)](-), in a murine model of liver tumor using a clinical magnetic resonance imaging scanner. The colon cancer cell lines with and without beta-glucuronidase (beta G) expression were implanted into the liver of mice. Self-synthesized gadolinium-based magnetic resonance contrast agent, [Gd(DOTA-FP beta G)], was administered to measure enhancement on magnetic resonance images using a commercially available agent, [Gd(DOTA)](-), as control in a clinical 3.0 tesla (T) magnetic resonance scanner. In vivo fluorescence imaging and histopathology of the liver were also performed to compare and correlate with the magnetic resonance studies. The in vivo fluorescence imaging failed to depict a sufficiently intense signal for liver or liver tumor of mice without exposure of the liver following an incision on the abdominal wall. The tissue-specific magnetic resonance agent, [Gd(DOTA-FP beta G)], caused significantly stronger enhancement in tumors expressing beta G (CT26/m beta G-eB7) than in tumors not expressing beta G (CT26) (p < 0.05). In the magnetic resonance imaging studies using control agent [Gd(DOTA)](-), the tumors with and without beta G expression depicted no significant difference in enhancement on the T1-weighted images. The [Gd(DOTA-FP beta G)] also provided significantly more contrast uptake in the CT26/m beta G-eB7 tumor than in the normal liver parenchyma, whereas the [Gd(DOTA)](-) did not. This study confirms that better contrast enhancement can be readily detected in vivo by the use of a tissue-specific magnetic resonance contrast agent to target tumor cells with specific biomarkers in a clinical magnetic resonance imaging scanner. Copyright (c) 2012, Kaohsiung Medical University. Published by Elsevier Taiwan LLC. All rights reserved.
URI: http://dx.doi.org/10.1016/j.kjms.2012.09.002
http://hdl.handle.net/11536/21902
ISSN: 1607-551X
DOI: 10.1016/j.kjms.2012.09.002
期刊: KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
Volume: 29
Issue: 5
起始頁: 246
結束頁: 253
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