Title: Tumor Marker Detection by Aptamer-Functionalized Graphene Oxide
Authors: Lin, Ming-Yu
Lu, Yen-Pei
Grumezescu, Alexandru Mihai
Ho, Fu Han
Kao, Ying-Hsien
Yang, Yuh-Shyong
Yang, Chih-Hui
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
Keywords: Aptamers;Biosensors;Functionalization;Graphene oxide;Platelet-derived growth factors;Tumor marker
Issue Date: 1-Jan-2013
Abstract: Interaction of the single-stranded DNA (ssDNA) aptamers and proteins was investigated on graphene oxide (GO)-based biosensors. GO was applied for biological applications because of its unique properties, which include water dispensability, exclusive selection of ssDNA, and fluorescence-quenching effects. In this study, aptamer-functionalized GO sheets of nanometer-scale thickness and micrometer-scale dimension were developed. Based on a simple mixed-and-measured strategy, platelet-derived growth factors (PDGF), the tumor marker of ovarian cancer, triggered aptamers conformation switched and deliberated aptamers from GO surface according to thermal dynamic analysis. By functionalizing with various aptamers on GO, the strategy could provide designed functions on GO-based biosensors, bioreactors, and other devices.
URI: http://dx.doi.org/10.2174/1385272811317020008
ISSN: 1385-2728
DOI: 10.2174/1385272811317020008
Volume: 17
Issue: 2
Begin Page: 132
End Page: 136
Appears in Collections:Articles