標題: A hybrid nanoparticle matrix for mass spectrometry
作者: Li, Po-Han
Huang, Shin-Yi
Chen, Yu-Chie
Urban, Pawel L.
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
公開日期: 2013
摘要: We demonstrate hybrid inorganic-organic nanoparticles -based on silicon dioxide and 9-aminoacridine- which can enhance mass spectrometric analysis and imaging in the negative-ion mode. The nanoparticles bind to the surface of cells while still in the liquid phase, which is followed by a controlled release of the organic matrix molecules triggered by alkaline ammonia vapours delivered to the specimen in a closed chamber. First, the method was optimized and tested using standard compounds as analytes. Second, the spatial resolution was verified using a natural fibre (diameter similar to 20 mu m). Third, the method was used in the imaging of molecules present in the outer layer of large algal cells (Closterium acerosum). An important advantage of this method is that nanoparticles loaded with organic matrix can be applied to the biological cells as aqueous suspensions; hence, the release and spreading of metabolites can be limited during sample preparation for MALDI-MS. In addition, since this method does not involve spraying, exposure of experimenters to toxic aerosols is decreased.
URI: http://hdl.handle.net/11536/21771
http://dx.doi.org/10.1039/c3ra22977g
ISSN: 2046-2069
DOI: 10.1039/c3ra22977g
期刊: RSC ADVANCES
Volume: 3
Issue: 19
起始頁: 6865
結束頁: 6870
顯示於類別:期刊論文


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  1. 000317929800027.pdf