標題: Association of poly(N-isopropylacrylamide) containing nucleobase multiple hydrogen bonding of adenine for DNA recognition
作者: Yang, Hsiu-Wen
Chen, Jem-Kun
Cheng, Chih-Chia
Kuo, Shiao-Wei
應用化學系
Department of Applied Chemistry
關鍵字: Supramolecule;Poly(N-isopropylacrylamide);Adenine;DNA recognition
公開日期: 15-四月-2013
摘要: In this study we used the poly(N-isopropylacrylamide) (PNIPAAm) as a medium to generate PNIPAAm-adenine supramolecular complexes. A nucleobase-like hydrogen bonding (NLHB) between PNIPAAm and adenine was found that changed the morphology, crystalline structure, and temperature responsiveness of PNIPAAm microgels relatively to the adenine concentrations. With increasing the adenine concentration, the PNIPAAm-adenine supramolecular complexes gradually altered their morphologies from microgel particles to thin film structures and suppressed the thermodynamical coil-to-globule transition of PNIPAAm because of the NLHB existed between the PNIPAAm amide and ester groups and the adenine amide groups (C=O center dot center dot center dot H-N and N-H center dot center dot center dot N-R), verified by FTIR spectral analysis. NLHB was also diverse and extensive upon increasing the temperature; therefore, the thermoresponsive behavior of the complexes was altered with the NLBH intensity, evaluated by the inter-association equilibrium constant (K-a) above and below their LCST. Therefore, PNIPAAm can be as a medium to recognize adenine in various concentrations, which could potentially be applied in DNA recognition. (C) 2013 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.apsusc.2013.01.074
http://hdl.handle.net/11536/21671
ISSN: 0169-4332
DOI: 10.1016/j.apsusc.2013.01.074
期刊: APPLIED SURFACE SCIENCE
Volume: 271
Issue: 
起始頁: 60
結束頁: 69
顯示於類別:期刊論文


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  1. 000316787400009.pdf