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dc.contributor.authorChen, Wen-Hungen_US
dc.contributor.authorHsu, I-Hsiangen_US
dc.contributor.authorSun, Yuh-Changen_US
dc.contributor.authorWang, Yu-Kuoen_US
dc.contributor.authorWu, Tung-Kungen_US
dc.date.accessioned2014-12-08T15:30:12Z-
dc.date.available2014-12-08T15:30:12Z-
dc.date.issued2013-05-03en_US
dc.identifier.issn0021-9673en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.chroma.2013.02.030en_US
dc.identifier.urihttp://hdl.handle.net/11536/21643-
dc.description.abstractA facile method for accurate detection of type I dengue virus (DV1) infection from complex biological mixtures, using type specific immunocapture coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), was developed. The biological mixtures were treated with magnetic particles coated with a monoclonal antibody directly against type 1 dengue virus. After immunocapture purification, the DV1 was eluted with 30% acetic acid, directly spotted with seed-layer method, and analyzed by MALDI-TOF MS for DV1 capsid protein. The detection limit of the assay was similar to 10(5) pfu/mL by MALDI-TOF MS. The immunocapture could unambiguously differentiate the DV1 from other serotypes of the dengue viruses and Japanese encephalitis virus, and could be used as a specific probe to detect DV1 from complex biological mixtures. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectImmunocaptureen_US
dc.subjectDengue virusen_US
dc.subjectMonoclonal antibodyen_US
dc.subjectMatrix-assisted laser desorption/ionization time-of-flight mass spectrometryen_US
dc.titleImmunocapture couples with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid detection of type 1 dengue virusen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.chroma.2013.02.030en_US
dc.identifier.journalJOURNAL OF CHROMATOGRAPHY Aen_US
dc.citation.volume1288en_US
dc.citation.issueen_US
dc.citation.spage21en_US
dc.citation.epage27en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000317707900003-
dc.citation.woscount3-
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