標題: Immunocapture couples with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid detection of type 1 dengue virus
作者: Chen, Wen-Hung
Hsu, I-Hsiang
Sun, Yuh-Chang
Wang, Yu-Kuo
Wu, Tung-Kung
生物科技學系
Department of Biological Science and Technology
關鍵字: Immunocapture;Dengue virus;Monoclonal antibody;Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
公開日期: 3-May-2013
摘要: A facile method for accurate detection of type I dengue virus (DV1) infection from complex biological mixtures, using type specific immunocapture coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), was developed. The biological mixtures were treated with magnetic particles coated with a monoclonal antibody directly against type 1 dengue virus. After immunocapture purification, the DV1 was eluted with 30% acetic acid, directly spotted with seed-layer method, and analyzed by MALDI-TOF MS for DV1 capsid protein. The detection limit of the assay was similar to 10(5) pfu/mL by MALDI-TOF MS. The immunocapture could unambiguously differentiate the DV1 from other serotypes of the dengue viruses and Japanese encephalitis virus, and could be used as a specific probe to detect DV1 from complex biological mixtures. (C) 2013 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.chroma.2013.02.030
http://hdl.handle.net/11536/21643
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2013.02.030
期刊: JOURNAL OF CHROMATOGRAPHY A
Volume: 1288
Issue: 
起始頁: 21
結束頁: 27
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