標題: Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy
作者: Chien, C. C.
Tseng, P. Y.
Chen, H. H.
Hua, T. E.
Chen, S. T.
Chen, Y. Y.
Leng, W. H.
Wang, C. H.
Hwu, Y.
Yin, G. C.
Liang, K. S.
Chen, F. R.
Chu, Y. S.
Yeh, H. I.
Yang, Y. C.
Yang, C. S.
Zhang, G. L.
Je, J. H.
Margaritondo, G.
電子物理學系
Department of Electrophysics
關鍵字: X-ray microscopy;Phase contrast radiology;Fresnel phase zone plate;Subcellular organelle
公開日期: 1-May-2013
摘要: Our experimental results demonstrate that full-field hard-X-ray microscopy is finally able to investigate the internal structure of cells in tissues. This result was made possible by three main factors: the use of a coherent (synchrotron) source of X-rays, the exploitation of contrast mechanisms based on the real part of the refractive index and the magnification provided by high-resolution Fresnel zone-plate objectives. We specifically obtained high-quality microradiographs of human and mouse cells with 29 nm Rayleigh spatial resolution and verified that tomographic reconstruction could be implemented with a final resolution level suitable for subcellular features. We also demonstrated that a phase retrieval method based on a wave propagation algorithm could yield good subcellular images starting from a series of defocused microradiographs. The concluding discussion compares cellular and subcellular hard-X-ray microradiology with other techniques and evaluates its potential impact on biomedical research. (C) 2012 Elsevier Inc. All rights reserved.
URI: http://dx.doi.org/10.1016/j.biotechadv.2012.04.005
http://hdl.handle.net/11536/21505
ISSN: 0734-9750
DOI: 10.1016/j.biotechadv.2012.04.005
期刊: BIOTECHNOLOGY ADVANCES
Volume: 31
Issue: 3
起始頁: 375
結束頁: 386
Appears in Collections:Conferences Paper


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