標題: Reconstitution of the early steps of gliotoxin biosynthesis in Aspergillus nidulans reveals the role of the monooxygenase GliC
作者: Chang, Shu-Lin
Chiang, Yi-Ming
Yeh, Hsu-Hua
Wu, Tung-Kung
Wang, Clay C. C.
生物科技學系
Department of Biological Science and Technology
關鍵字: Biosynthesis;Gliotoxin;Heterologous expression
公開日期: 1-Apr-2013
摘要: The gliotoxin, a member of the epipolythiodioxopiperazine (ETP), has received considerable attention from the scientific community for its wide range of biological activity. Despite the identification of gliotoxin cluster, however, the sequence of steps in the gliotoxin biosynthesis has remained elusive. As an alternative to the gene knock-out and biochemical approaches used so far, here we report using a heterologous expression approach to determine the sequence of the early steps of gliotoxin biosynthesis in Aspergillus nidulans. We identified the GliC, a monooxygenases that involved in the second step of gliotoxin biosynthesis pathway through the catalyzing the hydroxylation at the alpha-position of L-Phe. (C) 2013 Elsevier Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.bmcl.2013.01.099
http://hdl.handle.net/11536/21352
ISSN: 0960-894X
DOI: 10.1016/j.bmcl.2013.01.099
期刊: BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume: 23
Issue: 7
起始頁: 2155
結束頁: 2157
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