Title: Rhubarb inhibits hepatocellular carcinoma cell metastasis via GSK-3-beta activation to enhance protein degradation and attenuate nuclear translocation of beta-catenin
Authors: Tsai, Kun-Hsi
Hsien, Hau-Hsueh
Chen, Li-Mien
Ting, Wei-Jen
Yang, Yuh-Shyong
Kuo, Chia-Hua
Tsai, Chang-Hai
Tsai, Fuu-Jen
Tsai, Henry J.
Huang, Chih-Yang
Department of Biological Science and Technology
Keywords: Rhubarb;Hepatocellular carcinoma (HCC);GSK-3-beta;beta-Catenin
Issue Date: 1-May-2013
Abstract: The aim of our study was to investigate the mechanisms by which rhubarb regulates beta-catenin as well as metastasis of hepatocellular carcinomas. Our results revealed that rhubarb extract inhibited HA22T cell migration ability in wound healing, migration and invasion assays in a dose-dependent manner. Rhubarb also reduced beta-catenin protein level, downregulated its downstream proteins, cyclin D, Tbx3 and c-Myc, and attenuated the expression of MMP9 and contactin-1 metastatic factors. Additionally, rhubarb inhibited beta-catenin nuclear accumulation and induced its degradation via proteasome-mediated pathway. Furthermore, we found that rhubarb suppressed the p-ser(9) GSK-3-beta protein level to inactivate Wnt signalling and reduce beta-catenin protein level. Taken together; we found that rhubarb blocked the metastatic process of HA22T hepatocellular carcinoma cells mediated through GSK-3-beta activation, and enhancement of protein degradation as well as reduction of the nuclear accumulation of beta-catenin. (C) 2012 Elsevier Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.foodchem.2012.10.038
ISSN: 0308-8146
DOI: 10.1016/j.foodchem.2012.10.038
Volume: 138
Issue: 1
Begin Page: 278
End Page: 285
Appears in Collections:Articles

Files in This Item:

  1. 000314193800042.pdf