標題: Investigation of organelle-specific intracellular water structures with Raman microspectroscopy
作者: Tiwari, Shraeddha
Ando, Masahiro
Hamaguchi, Hiro-o
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: Raman microspectroscopy;water;living cells;imaging;multivariate analysis
公開日期: 1-二月-2013
摘要: The study of intracellular water in living cells is a challenge of fundamental importance. While the critical roles of water in maintaining and propagating life have been widely recognized and accepted, our understanding of water/biomolecule interactions is surprisingly limited. Using Raman microspectroscopic imaging of a living yeast cell followed by a multivariate analysis in form of the nonnegative matrix factorization method, we successfully resolve organelle-specific water structures. The intensities and the band profiles of the segregated water OH stretch spectra yield important and otherwise unobtainable information on the extensive effect of the water/biomolecule interactions in a given organelle on the hydrogen-bonding network of water molecules. Copyright (C) 2012 John Wiley & Sons, Ltd.
URI: http://dx.doi.org/10.1002/jrs.4165
http://hdl.handle.net/11536/21024
ISSN: 0377-0486
DOI: 10.1002/jrs.4165
期刊: JOURNAL OF RAMAN SPECTROSCOPY
Volume: 44
Issue: 2
起始頁: 167
結束頁: 169
顯示於類別:期刊論文


文件中的檔案:

  1. 000313993300001.pdf