標題: Improved DNA detection by utilizing electrically neutral DNA probe in field-effect transistor measurements as evidenced by surface plasmon resonance imaging
作者: Chen, Wen-Yih
Chen, Hon-Chen
Yang, Yuh-Shyong
Huang, Chun-Jen
Chan, Hardy Wai-Hong
Hu, Wen-Pin
生物科技學系
Department of Biological Science and Technology
關鍵字: SPR imaging;Field-effect transistor;Biosensors;Ethylated DNA;DNA analog
公開日期: 15-三月-2013
摘要: Intensive efforts have been focused on the development of ultrasensitive DNA biosensors capable of quantitative gene expression analysis. Various neutralized nucleic acids have been demonstrated as alternative and attractive probe for the design of a DNA chip. However, the mechanism of the improvements has not been clearly revealed. In this investigation, we used a newly developed neutral ethylated DNA (E-DNA), a DNA analog with the "RO-P-O" backbone (wherein R could be methyl, ethyl, aryl, or alkyl group) obtained from synthetic procedures, and a silicon nanowire (SiNW) field-effect transistor (FET) to evaluate the difference in DNA detection performance while using E-DNA and DNA as probes. It is demonstrated that using the E-DNA probe in the FET measurement could have a significantly enhanced effect upon the detection sensitivity. Surface plasmon resonance imaging (SPRi) was used to evidence the mechanism of the improved detection sensitivity. SPRi analysis showed the amounts of probe immobilization on the sensor surface and the hybridization efficiency were both enhanced with the use of E-DNA. Consequently, neutral ethylated DNA probe hold a great promise for DNA sensing, especially in the electrical-based sensor. (C) 2012 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.bios.2012.10.010
http://hdl.handle.net/11536/21000
ISSN: 0956-5663
DOI: 10.1016/j.bios.2012.10.010
期刊: BIOSENSORS & BIOELECTRONICS
Volume: 41
Issue: 
起始頁: 795
結束頁: 801
顯示於類別:期刊論文


文件中的檔案:

  1. 000314191300119.pdf