Title: Application of aurintricarboxylic acid for the adherence of mouse P19 neurons and primary hippocampal neurons to noncoated surface in serum-free culture
Authors: Lee, Tsung-Yih
Chen, Wen-Shin
Huang, Yung-An
Liu, Ting-Wei
Hwang, Eric
Tseng, Ching-Ping
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
Institute of Molecular Medicine and Bioengineering
Keywords: aurintricarboxylic acid;serum-free medium;P19 neuron cells;hippocampal neurons;cell attachment reagent
Issue Date: 1-Nov-2012
Abstract: Dissociated primary neuron culture has been the most widely used model systems for neuroscience research. Most of these primary neurons are cultured on adhesion matrix-coated surface to provide a proper environment for cell anchorage under serum-free conditions. In this study, we provide an alternative technique to promote the adhesions of these neurons using aurintricarboxylic acid (ATA), a nonpeptide compound, without surface manipulations. We first demonstrated that ATA could promote Chinese hamster ovary cell attachment and proliferation in serum-free medium in a dosage-dependent manner. We later showed that ATA significantly enhanced the attachment of the retinoic acid differentiated P19 mouse embryonal carcinoma (P19) neurons, with an optimal concentration around 30 mu g/mL. A similar result was seen in primary hippocampal neurons, with an optimal ATA concentration around 15 mu g/mL. Further morphological assessments revealed that the average neurite length and neuronal polarization were almost identical to that obtained using a conventional method with poly-L-lysine surface. The advantages of using the ATA treatment technique for immunochemical analysis are discussed. (C) 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012
URI: http://dx.doi.org/10.1002/btpr.1638
ISSN: 8756-7938
DOI: 10.1002/btpr.1638
Volume: 28
Issue: 6
Begin Page: 1566
End Page: 1574
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