標題: Control of growth and inflammatory response of macrophages and foam cells with nanotopography
作者: Mohiuddin, Mohammed
Pan, Hsu-An
Hung, Yao-Ching
Huang, Guewha Steven
材料科學與工程學系
Department of Materials Science and Engineering
關鍵字: Cell adhesion;Nanotopography;Macrophages;Foam cell;Biocompatible;Inflammatory response
公開日期: 16-七月-2012
摘要: Macrophages play an important role in modulating the immune function of the human body, while foam cells differentiated from macrophages with subsequent fatty streak formation play a key role in atherosclerosis. We hypothesized that nanotopography modulates the behavior and function of macrophages and foam cells without bioactive agent. In the present study, nanodot arrays ranging from 10aEuro to 200aEuronm were used to evaluate the growth and function of macrophages and foam cells. In the quantitative analysis, the cell adhesion area in macrophages increased with 10- to 50-nm nanodot arrays compared to the flat surface, while it decreased with 100- and 200-nm nanodot arrays. A similar trend of adhesion was observed in foam cells. Immunostaining, specific to vinculin and actin filaments, indicated that a 50-nm surface promoted cell adhesion and cytoskeleton organization. On the contrary, 200-nm surfaces hindered cell adhesion and cytoskeleton organization. Further, based on quantitative real-time polymerase chain reaction data, expression of inflammatory genes was upregulated for the 100- and 200-nm surfaces in macrophages and foam cells. This suggests that nanodots of 100aEuroaEuro parts per thousand and 200aEuronm triggered immune inflammatory stress response. In summary, nanotopography controls cell morphology, adhesions, and proliferation. By adjusting the nanodot diameter, we could modulate the growth and expression of function-related genes in the macrophages and foam cell system. The nanotopography-mediated control of cell growth and morphology provides potential insight for designing cardiovascular implants.
URI: http://dx.doi.org/10.1186/1556-276X-7-394
http://hdl.handle.net/11536/20701
ISSN: 1931-7573
DOI: 10.1186/1556-276X-7-394
期刊: NANOSCALE RESEARCH LETTERS
Volume: 7
Issue: 
起始頁: 1
結束頁: 9
顯示於類別:期刊論文


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