Title: Human Serum Albumin Stabilized Gold Nanoclusters as Selective Luminescent Probes for Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus
Authors: Chan, Po-Han
Chen, Yu-Chie
Department of Applied Chemistry
Issue Date: 6-Nov-2012
Abstract: In this work, human serum albumin (HSA) stabilized gold nanoclusters (HSA-AuNCs) with reddish photoluminescence were used as sensing probes for pathogenic bacteria including Enterobacter cloacae, Escherichia coli J96, Pseudomonas aeruginosa, pandrug-resistant Acinetobacter baumannii (PDRAB), Staphylococcus aureus, methicillin-resistant S. aureus (MASA), Streptococcus pyogenes, and vancomycin-resistant Enterococcus faecalis (VRE). We discovered that HSA-AuNCs have unique affinity with S. aureus and MASA In addition to demonstrating the selective sensing ability of HSA-AuNCs toward S. aureus and MRSA, the binding peptide motifs identified from HSA-AuNCs were characterized by mass spectrometry. The identified binding peptides were further used as the reducing and stabilizing agents for generation of peptide-bound AuNCs (Pep-AuNCs). The generated Pep-AuNCs were demonstrated to have the binding affinities with S. aureus and MRSA.
URI: http://dx.doi.org/10.1021/ac302417k
ISSN: 0003-2700
DOI: 10.1021/ac302417k
Volume: 84
Issue: 21
Begin Page: 8952
End Page: 8956
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