Title: Preparation of High-Performance Water-Soluble Quantum Dots for Biorecognition through Fluorescence Resonance Energy Transfer
Authors: Huang, Chin-Ping
Chao, Cheng-Fu
Shen, Mo-Yuan
Chen, Teng-Ming
Li, Yaw-Kuen
Department of Applied Chemistry
Keywords: FRET;molecular recognition;photoactivation;quantum dots;steroids
Issue Date: 1-Dec-2012
Abstract: An improved method for the synthesis of high-performance and water-soluble quantum dots (QDs) involving the encapsulation of mercaptosuccinic acid coated QDs (MSA-QDs) with poly(diallyldimethylammonium chloride) (PDDA) followed by their direct photoactivation with fluorescent radiation near 295 K to yield PDDA-coated QDs (PDDA-QDs) has been demonstrated. The quantum yield (QY) of the PDDA-QDs was significantly improved from 0.6 (QY of MSA-QDs) to 48?%. By using this synthetic strategy, highly photoluminescent PDDA-QDs of varied size were readily prepared. The surface properties of PDDA-QDs and MSA-QDs were extensively characterized. The highly luminescent and positively charged PDDA-QDs serve as a useful and convenient tool for protein adsorption. With a ?5-3-ketosteroid isomerase adsorbed PDDA-QD complex, the biorecognition of steroids was demonstrated through the application of fluorescent resonance energy transfer.
URI: http://dx.doi.org/10.1002/asia.201200526
ISSN: 1861-4728
DOI: 10.1002/asia.201200526
Volume: 7
Issue: 12
Begin Page: 2848
End Page: 2853
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