標題: The difference in myocardial injuries and mitochondrial damages between asphyxial and ventricular fibrillation cardiac arrests
作者: Tsai, Min-Shan
Huang, Chien-Hua
Tsai, Shang-Ho
Tsai, Chia-Ying
Chen, Huei-Wen
Cheng, Hsaio-Ju
Hsu, Chiung-Yuan
Wang, Tzung-Dau
Chang, Wei-Tien
Chen, Wen-Jone
電子工程學系及電子研究所
電機工程學系
Department of Electronics Engineering and Institute of Electronics
Department of Electrical and Computer Engineering
公開日期: 1-十月-2012
摘要: Introduction: Ventricular fibrillation (VF) and asphyxia account for most cardiac arrests but differ in cardiac arrest course, neurologic deficit, and myocardial damage. In VF resuscitation, cardiac mitochondria were known to be damaged via excess generation of reactive oxygen species. This study evaluated the difference of cardiac mitochondrial damages between VF and asphyxial cardiac arrests. Methods: In the VF + electrical shock (ES) group, VF was induced and untreated for 5 minutes, followed by 1 minute of cardiopulmonary resuscitation (CPR) and 1 ES of 5 J. Animals were killed immediately after ES. In the asphyxia group, cardiac arrest was induced by airway obstruction, and then pulselessness was maintained for 5 minutes, followed by 1 minute of CPR. The animals were killed immediately after CPR. The histology and ultrastructural changes of myocardium and complex activities and respiration of mitochondria were evaluated. The mitochondrial permeability transition pore opening was measured based on mitochondrial swelling rate. Results: The histopathologic examinations showed myocardial necrosis and mitochondrial damage in both cardiac arrests. Instead of regional damages of myocardium in the VF + ES group, the myocardial injury in the asphyxia group distributed diffusely. The asphyxia group demonstrated more severe mitochondrial damage than the VF + ES group, which had a faster mitochondrial swelling rate, more decreased cytochrome c oxidase activity, and more impaired respiration. Conclusions: Both VF and asphyxial cardiac arrests caused myocardial injuries and mitochondrial damages. Asphyxial cardiac arrest presented more diffuse myocardial injuries and more severe mitochondrial damages than VF cardiac arrest. (c) 2012 Elsevier Inc. All rights reserved.
URI: http://dx.doi.org/10.1016/j.ajem.2012.01.001
http://hdl.handle.net/11536/20449
ISSN: 0735-6757
DOI: 10.1016/j.ajem.2012.01.001
期刊: AMERICAN JOURNAL OF EMERGENCY MEDICINE
Volume: 30
Issue: 8
起始頁: 1540
結束頁: 1548
顯示於類別:期刊論文


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