標題: Protein Engineering of Saccharomyces cerevisiae Oxidosqualene-Lanosterol Cyclase into Parkeol Synthase
作者: Liu, Yuan-Ting
Hu, Tain-Chang
Chang, Cheng-Hsiang
Shie, Wen-Shiang
Wu, Tung-Kung
生物科技學系
Department of Biological Science and Technology
公開日期: 19-Oct-2012
摘要: A Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase mutant, ERG7(T384Y/Q450H/V4541), produced parkeol but not lanosterol as the sole end product. Parkeol undergoes downstream metabolism to generate compounds 9 and 10. In vitro incubation of parkeol produced a product profile similar to that of the in vivo experiment. In summary, parkeol undergoes a metabolic pathway similar to that of cycloartenol in yeast but distinct from that of lanosterol in yeast, suggesting that two different metabolic pathways of postoxidosqualene cyclization may exist in S. cerevisiae.
URI: http://dx.doi.org/10.1021/ol302341h
http://hdl.handle.net/11536/20423
ISSN: 1523-7060
DOI: 10.1021/ol302341h
期刊: ORGANIC LETTERS
Volume: 14
Issue: 20
起始頁: 5222
結束頁: 5225
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