標題: Efficacy verification and microscopic observations of an anticancer peptide, CB1a, on single lung cancer cell
作者: Kao, Feng-Sheng
Pan, Yun-Ru
Hsu, Ray-Quen
Chen, Hueih-Min
機械工程學系
Department of Mechanical Engineering
關鍵字: Atomic force microscopy;CB1a, single cell;Cancer cell;Anticancer peptide
公開日期: 1-十二月-2012
摘要: In this work, we introduce a new customized anti-lung cancer peptide, CB1a, with IC50 of about 25.0 +/- 1.6 mu M on NCI-H460 lung cancer cells. Using a multi-cellular tumor spheroid (MCTS) model, results show that CB1a is potent in preventing the growth of lung cancer tumor-like growths in vitro. Additionally, atomic force microscopy (AFM) was used to examine cell surface damage of a single cancer. The mechanism for cell death under CB1a toxicity was verified as being largely due to cell surface damage. Moreover, with a treatment dosage of CB1a at 25 mu M, Young's module (E) shows that the elasticity and stiffness of cancer cell decreased with time such that the interaction time for a 50% reduction of E (IT50) was about 7.0 min. This new single-cell toxicity investigation using IT50 under AFM assay can be used to separately verify drug efficacy in support of the traditional IC50 measurement in bulk solution. These results could be of special interest to researchers engaged in new drug development. (C) 2012 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.bbamem.2012.07.019
http://hdl.handle.net/11536/20394
ISSN: 0005-2736
DOI: 10.1016/j.bbamem.2012.07.019
期刊: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume: 1818
Issue: 12
起始頁: 2927
結束頁: 2935
顯示於類別:期刊論文


文件中的檔案:

  1. 000309801500001.pdf