標題: A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis
作者: Kao, Chien-Han
Cheng, Chiu-Min
Chuang, Kuo-Hsiang
Chuang, Chih-Hung
Tzou, Shey-Cherng
Cheng, Ta-Chun
Hsieh, Yuan-Chin
Liao, Kuang-Wen
Wang, Yun-Ming
Chang, Long-Sen
Roffler, Steve R.
Chen, Fang Ming
Cheng, Tian-Lu
生物科技學系
電子工程學系及電子研究所
Department of Biological Science and Technology
Department of Electronics Engineering and Institute of Electronics
關鍵字: 14-Epitope tag protein ladder;Epitope tags;Anti-epitope tag antibodies;Protein molecular estimation;Sodium dodecyl sulfate-polyacrylamide gel electrophoresis;Western blot analysis
公開日期: 1-十二月-2012
摘要: We designed a protein ladder (hereafter referred to as "Mega-tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R-2 = 0.997 +/- 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis. (c) 2012 Elsevier Inc. All rights reserved.
URI: http://dx.doi.org/10.1016/j.ab.2012.08.001
http://hdl.handle.net/11536/20389
ISSN: 0003-2697
DOI: 10.1016/j.ab.2012.08.001
期刊: ANALYTICAL BIOCHEMISTRY
Volume: 431
Issue: 1
起始頁: 1
結束頁: 3
顯示於類別:期刊論文


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  1. 000310496200001.pdf