標題: Differential adhesion and actomyosin cable collaborate to drive Echinoid-mediated cell sorting
作者: Chang, Li-Hsun
Chen, Peilong
Lien, Mong-Ting
Ho, Yu-Huei
Lin, Chiao-Ming
Pan, Yi-Ting
Wei, Shu-Yi
Hsu, Jui-Chou
生物科技學系
Department of Biological Science and Technology
關鍵字: Echinoid;Differential adhesion;Differential expression;Cell sorting;Actomyosin;Drosophila
公開日期: 1-九月-2011
摘要: Cell sorting involves the segregation of two cell populations into 'immiscible' adjacent tissues with smooth borders. Echinoid (Ed), a nectin ortholog, is an adherens junction protein in Drosophila, and cells mutant for ed sort out from the surrounding wild-type cells. However, it remains unknown which factors trigger cell sorting. Here, we dissect the sequence of this process and find that cell sorting occurs when differential expression of Ed triggers the assembly of actomyosin cable. Conversely, Ed-mediated cell sorting can be rescued by recruitment of Ed, via homophilic or heterophilic interactions, to the wild-type cell side of the clonal interface, even when differential Ed expression persists. We found, unexpectedly, that when actomyosin cable was largely absent, differential adhesion was sufficient to cause limited cell segregation but with a jagged tissue border (imperfect sorting). We propose that Ed-mediated cell sorting is driven both by differential Ed adhesion that induces cell segregation with a jagged border and by actomyosin cable assembly at the interface that smoothens this border.
URI: http://dx.doi.org/10.1242/dev.062257
http://hdl.handle.net/11536/19971
ISSN: 0950-1991
DOI: 10.1242/dev.062257
期刊: DEVELOPMENT
Volume: 138
Issue: 17
起始頁: 3803
結束頁: 3812
顯示於類別:期刊論文


文件中的檔案:

  1. 000293687700019.pdf