標題: Resolution studies on counter-current chromatography using supercritical fluid carbon dioxide
作者: Yu, T
Chen, YH
應用化學系
Department of Applied Chemistry
關鍵字: counter-current chromatography;mobile phase composition;resolution;naphthalene;benzophenone;acetophenone
公開日期: 28-十一月-1997
摘要: Counter-current chromatography (CCC) is a form of Liquid-liquid partition chromatography. It requires two immiscible solvent phases; the stationary phase is retained in thr separation column, generally by centrifugal force, while the mobile phase is eluted. We recently replaced the mobile phase with supercritical fluid carbon dioxide (SF CO2). Since the solvent strength of SF CO2 can be varied by changing the temperature and pressure of the system, separation adjustments are thus more versatile. We investigated the pressure and temperature effects on resolution using water and low-carbon alcohol mixtures as the stationary phases. It was demonstrated that these special properties of SF CO2 were indeed beneficial to the optimization of separations. In addition, the phase retention ratio was examined in terms of separation resolution. The results appeared very similar to those obtained from conventional traditional CCC. This study should be helpful for the future development of SF applications in CCC. (C) 1997 Elsevier Science B.V.
URI: http://dx.doi.org/10.1016/S0021-9673(97)00746-2
http://hdl.handle.net/11536/184
ISSN: 0021-9673
DOI: 10.1016/S0021-9673(97)00746-2
期刊: JOURNAL OF CHROMATOGRAPHY A
Volume: 790
Issue: 1-2
起始頁: 31
結束頁: 39
顯示於類別:期刊論文


文件中的檔案:

  1. 000070982700004.pdf