標題: Regulation of fumarase (fumB) gene expression in Escherichia coli in response to oxygen, iron and heme availability: role of the arcA, fur, and hemA gene products
作者: Tseng, CP
生物科技學系
Department of Biological Science and Technology
關鍵字: fumarase gene expression;fumB;aerobic vs. anaerobic growth;Escherichia coli
公開日期: 1-十二月-1997
摘要: Three distinct fumarases, FumA, FumB and FumC, have been reported in Escherichia coli. While the fumA and fumC gene products are expressed under aerobic cell growth conditions, the FumB fumarase appears to be more abundant during anaerobic growth. To study the transcriptional regulation of the fumB gene, a fumB-lacZ operon fusion was constructed and analyzed in a single copy under a variety of cell culture conditions. Expression of fumB-lacZ was fourfold higher under anaerobic than aerobic growth conditions. This anaerobic response is modulated by the ArcA and Fnr proteins, which function independently as anaerobic activators of fumB gene expression. Cellular iron limitation in a fur mutant caused fumB-lacZ expression to decrease sevenfold while cellular heme limitation decreased fumB gene expression twofold. In addition, fumB-lacZ expression was shown to vary depending on the DNA superhelicity. This study further delineates the regulation of the fumB gene in cell growth.
URI: http://dx.doi.org/10.1016/S0378-1097(97)00455-2
http://hdl.handle.net/11536/173
ISSN: 0378-1097
DOI: 10.1016/S0378-1097(97)00455-2
期刊: FEMS MICROBIOLOGY LETTERS
Volume: 157
Issue: 1
起始頁: 67
結束頁: 72
顯示於類別:期刊論文


文件中的檔案:

  1. 000072333600011.pdf