Title: Nanosurface design of dental implants for improved cell growth and function
Authors: Pan, Hsu-An
Hung, Yao-Ching
Chiou, Jin-Chern
Tai, Shih-Ming
Chen, Hsin-Hung
Huang, G. Steven
Department of Materials Science and Engineering
Institute of Electrical and Control Engineering
Issue Date: 24-Aug-2012
Abstract: A strategy was proposed for the topological design of dental implants based on an in vitro survey of optimized nanodot structures. An in vitro survey was performed using nanodot arrays with dot diameters ranging from 10 to 200 nm. MG63 osteoblasts were seeded on nanodot arrays and cultured for 3 days. Cell number, percentage undergoing apoptotic-like cell death, cell adhesion and cytoskeletal organization were evaluated. Nanodots with a diameter of approximately 50 nm enhanced cell number by 44%, minimized apoptotic-like cell death to 2.7%, promoted a 30% increase in microfilament bundles and maximized cell adhesion with a 73% increase in focal adhesions. An enhancement of about 50% in mineralization was observed, determined by von Kossa staining and by Alizarin Red S staining. Therefore, we provide a complete range of nanosurfaces for growing osteoblasts to discriminate their nanoscale environment. Nanodot arrays present an opportunity to positively and negatively modulate cell behavior and maturation. Our results suggest a topological approach which is beneficial for the design of dental implants.
URI: http://dx.doi.org/10.1088/0957-4484/23/33/335703
ISSN: 0957-4484
DOI: 10.1088/0957-4484/23/33/335703
Volume: 23
Issue: 33
End Page: 
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