Title: A piezoelectric immunosensor for specific capture and enrichment of viable pathogens by quartz crystal microbalance sensor, followed by detection with antibody-functionalized gold nanoparticles
Authors: Guo, Xiao
Lin, Chih-Sheng
Chen, Sz-Hau
Ye, Rebecca
Wu, Vivian C. H.
Department of Biological Science and Technology
Keywords: Biosensor;Foodborne pathogen;Gold nanoparticles;Quartz crystal microbalance
Issue Date: 1-Oct-2012
Abstract: A sensitive bacteria enrichment and detection system for viable Escherichia coil O157:H7 was developed using a piezoelectric biosensor-quartz crystal microbalance (QCM) with antibody-functionalized gold nanoparticles (AuNPs) used as detection verifiers and amplifiers. In the circulating-flow QCM system, capture antibodies for E. coil 0157:H7 were first immobilized onto the QCM chip. The sample containing E. coli 0157:H7 was circulated through the system in the presence of 10 ml of brain heart infusion (BHI) broth for 18 h. The cells of E. coli 0157:H7 specifically captured and enriched on the chip surface of the QCM were identified by QCM frequency changes. Listeria monocytogenes and Salmonella Typhimurium were used as negative controls. After bacterial enrichment, detection antibody-functionalized AuNPs were added to enhance the changes in detection signal. The use of BHI enrichment further enhanced the sensitivity of the developed system, achieving a detection limit of 0-1 log CFU/ml or g. The real-time monitoring method for viable E. coli 0157:H7 developed in this study can be used to enrich and detect viable cells simultaneously within 24 h. The unique advantages of the system developed offer great potential in the microbial analysis of food samples in routine settings. (C) 2012 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.bios.2012.05.024
ISSN: 0956-5663
DOI: 10.1016/j.bios.2012.05.024
Volume: 38
Issue: 1
Begin Page: 177
End Page: 183
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