標題: Activation of rabbit TLR9 by different CpG-ODN optimized for mouse and human TLR9
作者: Liu, Jin
Xu, Congfeng
Liu, Yi-Ling
Matsuo, Hanako
Hsieh, Rebecca Pe-Feng
Lo, Jeng-Fan
Tseng, Ping-Hui
Yuan, Chiun-Jye
Luo, Yunping
Xiang, Rong
Chuang, Tsung-Hsien
生物科技學系
Department of Biological Science and Technology
關鍵字: Immune adjuvant;Pattern recognition receptor;Toll-like receptor 9;CpG-ODN;Nuclear factor-kappa B
公開日期: 1-Sep-2012
摘要: "Synthetic CpG-oligodeoxynucleotides (CpG-ODN) are potent adjuvants that accelerate and boost antigen-specific immune responses. Toll-like receptor 9 (TLR9) is the cellular receptor for these CpG-ODN. Previous studies have shown species-specific activation of mouse TLR9 (mTLR9) and human TLR9 (hTLR9) by their optimized CpG-ODN. The interaction between rabbit TLR9 (rabTLR9) and CpG-ODN, however, has not been previously investigated. Here, we cloned and characterized rabTLR9 and comparatively investigated the activation of the rabbit, mouse, and human TLR9 by CpG-ODN. The complete open reading frame of rabTLR9 encodes 1028 amino acid residues, which share 70.6% and 75.5% of the identities of mTLR9 and hTLR9, respectively. Rabbit TLR9 is preferentially expressed in immune cells rich tissues, and is localized in intracellular vesicles. While mTLR9 and hTLR9 displayed species-specific recognition of their optimized CpG-ODN, rabbit TLR9 was activated by these CpG-ODN without any preference. This result suggests that rabTLR9 has a broader ligand-recognition profile than mouse and human TLR9. (C) 2012 Elsevier Ltd. All rights reserved."
URI: http://hdl.handle.net/11536/16716
ISSN: 0147-9571
期刊: COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
Volume: 35
Issue: 5
結束頁: 443
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