標題: Capulet and Slingshot share overlapping functions during Drosophila eye morphogenesis
作者: Lin, Chiao-Ming
Lin, Pei-Yi
Li, Yu-Chiao
Hsu, Jui-Chou
生物科技學系
Department of Biological Science and Technology
關鍵字: Capulet;Slingshot;Twinstar;F-actin;Eye development
公開日期: 1-Jan-1970
摘要: "Background: CAP/Capulet (Capt), Slingshot (Ssh) and Cofilin/Twinstar (Tsr) are actin-binding proteins that restrict actin polymerization. Previously, it was shown that low resolution analyses of loss-of-function mutations in capt, ssh and tsr all show ectopic F-actin accumulation in various Drosophila tissues. In contrast, RNAi depletion of capt, tsr and ssh in Drosophila S2 cells all affect actin-based lamella formation differently. Whether loss of these three related genes might cause the same effect in the same tissue remains unclear. Methods: Loss-of-function mutant clones were generated using the MARCM or EGUF system whereas overexpression clones were generated using the Flip-out system. Immunostaining were then performed in eye imaginal discs with clones. FRAP was performed in cultured eye discs. Results: Here, we compared their loss-of-function phenotype at single-cell resolution, using a sheet of epithelial cells in the Drosophila eye imaginal disc as a model system. Surprisingly, we found that capt and ssh, but not tsr, mutant cells within and posterior to the morphogenetic furrow (MF) shared similar phenotypes. The capt/ssh mutant cells possessed: (1) hexagonal cell packing with discontinuous adherens junctions; and (2) largely complementary accumulation of excessive phosphorylated myosin light chain (p-MLC) and F-actin rings at the apical cortex. We further showed that the capt/ssh mutant phenotypes depended on the inactivation of protein kinase A (PKA) and activation of Rho. Conclusions: Although Capt, Ssh and Tsr were reported to negatively regulate actin polymerization, we found that Capt and Ssh, but not Tsr, share overlapping functions during eye morphogenesis."
URI: http://dx.doi.org/46
http://hdl.handle.net/11536/16667
ISSN: 1021-7770
DOI: 46
期刊: JOURNAL OF BIOMEDICAL SCIENCE
Volume: 19
Issue: 
結束頁: 
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