標題: Let-7b is a novel regulator of hepatitis C virus replication
作者: Cheng, Ju-Chien
Yeh, Yung-Ju
Tseng, Ching-Ping
Hsu, Sheng-Da
Chang, Yu-Ling
Sakamoto, Naoya
Huang, Hsien-Da
生物科技學系
生物資訊及系統生物研究所
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
關鍵字: microRNA;Let-7b;HCV
公開日期: 1-八月-2012
摘要: "The non-coding microRNA (miRNA) is involved in the regulation of hepatitis C virus (HCV) infection and offers an alternative target for developing anti-HCV agent. In this study, we aim to identify novel cellular miRNAs that directly target the HCV genome with anti-HCV therapeutic potential. Bioinformatic analyses were performed to unveil liver-abundant miRNAs with predicted target sequences on HCV genome. Various cell-based systems confirmed that let-7b plays a negative role in HCV expression. In particular, let-7b suppressed HCV replicon activity and down-regulated HCV accumulation leading to reduced infectivity of HCVcc. Mutational analysis identified let-7b binding sites at the coding sequences of NS5B and 5'-UTR of HCV genome that were conserved among various HCV genotypes. We further demonstrated that the underlying mechanism for let-7b-mediated suppression of HCV RNA accumulation was not dependent on inhibition of HCV translation. Let-7b and IFN alpha-2a also elicited a synergistic inhibitory effect on HCV infection. Together, let-7b represents a novel cellular miRNA that targets the HCV genome and elicits anti-HCV activity. This study thereby sheds new insight into understanding the role of host miRNAs in HCV pathogenesis and to developing a potential anti-HCV therapeutic strategy."
URI: http://hdl.handle.net/11536/16614
ISSN: 1420-682X
期刊: CELLULAR AND MOLECULAR LIFE SCIENCES
Volume: 69
Issue: 15
結束頁: 2621
顯示於類別:期刊論文


文件中的檔案:

  1. 000306335000013.pdf