標題: Cancer cell labeling and tracking using fluorescent and magnetic nanodiamond
作者: Lien, Zhi-Yi
Hsu, Tzu-Chia
Liu, Kuang-Kai
Liao, Wei-Siang
Hwang, Kuo-Chu
Chao, Jui-I.
生物科技學系
分子醫學與生物工程研究所
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
關鍵字: Fluorescent and magnetic nanodiamond;Nanodiamond-bearing cancer cells;Flow cytometer;Magnetic device;Confocal microscope
公開日期: 1-九月-2012
摘要: "Nanodiamond, a promising carbon nanomaterial, develops for biomedical applications such as cancer cell labeling and detection. Here, we establish the nanodiamond-bearing cancer cell lines using the fluorescent and magnetic nanodiamond (FMND). Treatment with FMND particles did not significantly induce cytotoxicity and growth inhibition in HFL-1 normal lung fibroblasts and A549 lung cancer cells. The fluorescence intensities and particle complexities were increased in a time- and concentration-dependent manner by treatment with FMND particles in lung cancer cells; however, the existence of FMND particles inside the cells did not alter cellular size distribution. The FMND-bearing lung cancer cells could be separated by the fluorescent and magnetic properties of FMNDs using the flow cytometer and magnetic device, respectively. The FMND-bearing cancer cells were identified by the existence of FMNDs using flow cytometer and confocal microscope analysis. More importantly, the cell morphology, viability, growth ability and total protein expression profiles in the FMND-bearing cells were similar to those of the parental cells. The separated FMND-bearing cells with various generations were cryopreservation for further applications. After re-thawing the FMND-bearing cancer cell lines, the cells still retained the cell survival and growth ability. Additionally, a variety of human cancer types including colon (RKO), breast (MCF-7), cervical (HeLa), and bladder (BFTC905) cancer cells could be used the same strategy to prepare the FMND-bearing cancer cells. These results show that the FMND-bearing cancer cell lines, which reserve the parental cell functions, can be applied for specific cancer cell labeling and tracking. (C) 2012 Elsevier Ltd. All rights reserved."
URI: http://hdl.handle.net/11536/16584
ISSN: 0142-9612
期刊: BIOMATERIALS
Volume: 33
Issue: 26
結束頁: 6172
顯示於類別:期刊論文


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  1. 000306720400021.pdf