標題: Investigation of the cerebral hemodynamic response function in single blood vessels by functional photoacoustic microscopy
作者: Liao, Lun-De
Lin, Chin-Teng
Shih, Yen-Yu I.
Lai, Hsin-Yi
Zhao, Wan-Ting
Duong, Timothy Q.
Chang, Jyh-Yeong
Chen, You-Yin
Li, Meng-Lin
電機工程學系
腦科學研究中心
Department of Electrical and Computer Engineering
Brain Research Center
關鍵字: functional photoacoustic microscopy;electrical stimulation;total hemoglobin concentration;hemoglobin oxygen saturation
公開日期: 1-Jun-2012
摘要: The specificity of the hemodynamic response function (HRF) is determined spatially by the vascular architecture and temporally by the evolution of hemodynamic changes. Here, we used functional photoacoustic microscopy (fPAM) to investigate single cerebral blood vessels of rats after left forepaw stimulation. In this system, we analyzed the spatiotemporal evolution of the HRFs of the total hemoglobin concentration (HbT), cerebral blood volume (CBV), and hemoglobin oxygen saturation (SO2). Changes in specific cerebral vessels corresponding to various electrical stimulation intensities and durations were bilaterally imaged with 36 x 65-mu m(2) spatial resolution. Stimulation intensities of 1, 2, 6, and 10 mA were applied for periods of 5 or 15 s. Our results show that the relative functional changes in HbT, CBV, and SO2 are highly dependent not only on the intensity of the stimulation, but also on its duration. Additionally, the duration of the stimulation has a strong influence on the spatiotemporal characteristics of the HRF as shorter stimuli elicit responses only in the local vasculature (smaller arterioles), whereas longer stimuli lead to greater vascular supply and drainage. This study suggests that the current fPAM system is reliable for studying relative cerebral hemodynamic changes, as well as for offering new insights into the dynamics of functional cerebral hemodynamic changes in small animals. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [ DOI: 10.1117/1.JBO.17.6.061210]
URI: http://dx.doi.org/061210
http://hdl.handle.net/11536/16506
ISSN: 1083-3668
DOI: 061210
期刊: JOURNAL OF BIOMEDICAL OPTICS
Volume: 17
Issue: 6
結束頁: 
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