標題: Study of the reactive excited-state dynamics of delipidated bacteriorhodopsin upon surfactant treatments
作者: Cheng, Chi-Wen
Lee, Yuan-Pern
Chu, Li-Kang
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
公開日期: 29-Jun-2012
摘要: The dynamics of the reactive excited-state of bacteriorhodopsin treated with various surfactants have been investigated through the measurement of pump-probe absorption spectra. Upon excitation of bR at 570 nm in the presence of ionic surfactants CTAB and SDS, the lifetime of the reactive excited-state of the all-trans protonated Schiff base, probed at 460 nm, was observed to increase up to 20%, whereas an insignificant change was observed upon addition of neutral surfactant C6E2. Measurements of steady-state absorption spectra, fluorescence, and circular dichroism indicated that the bR suspensions retain their trimeric configuration with partial delipidation. This removal of lipids causes a structural alteration and the varied excited-state dynamics. (C) 2012 Elsevier B. V. All rights reserved.
URI: http://hdl.handle.net/11536/16464
ISSN: 0009-2614
期刊: CHEMICAL PHYSICS LETTERS
Volume: 539
Issue: 
結束頁: 151
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