標題: Using Gold Nanoclusters As Selective Luminescent Probes for Phosphate-Containing Metabolites
作者: Li, Po-Han
Lin, Ju-Yu
Chen, Cheng-Tai
Ciou, Wei-Ru
Chan, Po-Han
Luo, Liyang
Hsu, Hung-Yu
Diau, Eric Wei-Guang
Chen, Yu-Chie
應用化學系
Department of Applied Chemistry
公開日期: 3-七月-2012
摘要: Glutathione-bound gold nanoclusters (AuNCs@GSH) can emit reddish photoluminescence under illumination of ultraviolet light. The luminescence of the AuNCs@GSH is quenched when chelating with iron ions (AuNCs@GSH-Fe3+), presumably resulting from the effective electron transfer between the nanoclusters and iron ions. Nevertheless, we found that the luminescence of the gold nanoclusters can be restored in the presence of phosphate-containing molecules, which suggested the possibility of using AuNCs@GSH-Fe3+ complexes as the selective luminescent switches for phosphate-containing metabolites. Phosphate-containing metabolites such as adenosine-5'-triphosphate (ATP) and pyrophosphate play an important role in biological systems. In this study, we demonstrated that the luminescence of the AuNCs@GSH-Fe3+ is switched-on when mixing with ATP and pyrophosphate, which can readily be observed by the naked eye. It results from the high formation constants between phosphates and iron ions. When employing fluorescence spectroscopy as the detection tool, quantitative analysis for phosphate-containing metabolites such as ATP and pyrophosphate can be conducted. The linear range for ATP and pyrophosphate is 50 mu M to sub-millimolar, while the limit of detection for ATP and pyrophosphate are similar to 43 and similar to 28 mu M, respectively. Additionally, we demonstrated that the luminescence of the AuNCs@GSH-Fe3+ can also be turned on in the presence of phosphate-containing metabolites from cell lysates and blood plasma.
URI: http://hdl.handle.net/11536/16439
ISSN: 0003-2700
期刊: ANALYTICAL CHEMISTRY
Volume: 84
Issue: 13
結束頁: 5484
顯示於類別:期刊論文


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