Title: Detection of Gliadin in Foods Using a Quartz Crystal Microbalance Biosensor That Incorporates Gold Nanoparticles
Authors: Chu, Pei-Tzu
Lin, Chih-Sheng
Chen, Wei-Jung
Chen, Chih-Feng
Wen, Hsiao-Wei
Department of Biological Science and Technology
Keywords: gliadin;quartz crystal microbalance immunosensor;gold nanoparticles
Issue Date: 4-Jul-2012
Abstract: This work develops a label-free gliadin immunosensor that is based on changes in the frequency of a quartz crystal microbalance (QCM) chip. A higher sensitivity was obtained by applying 25 nm gold nanoparticles (AuNPs) to the surface of a bare QCM electrode. Subsequently, chicken anti-gliadin antibodies (IgY) were immobilized directly on the AuNP-modified surface by cross-linking amine groups in IgY with glutaraldehyde. Experimental results revealed that the change in frequency exhibited when 2 ppm gliadin was bound to the AuNP-modified electrode was 35 Hz (48%) greater than that of the bare gold electrode. The linear dynamic range in 60% ethanol was from 1 X 10(1) to 2 X 10(5) ppb gliadin, and the calculated limit of detection (LOD) was 8 ppb. The entire detection process was completed in 40 min and was highly repeatable. Additionally, the AuNP-modified QCM system generated results in the detection of gliadin in 10 commercial food products that were consistent with those obtained using an AOAC-approved gliadin kit. In conclusion, the QCM platform provides a potential alternative means of ensuring that people with wheat allergies and celiac patients have access to gliadin-free food.
URI: http://hdl.handle.net/11536/16438
ISSN: 0021-8561
Volume: 60
Issue: 26
End Page: 6483
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