Title: Transcranial imaging of functional cerebral hemodynamic changes in single blood vessels using in vivo photoacoustic microscopy
Authors: Liao, Lun-De
Lin, Chin-Teng
Shih, Yen-Yu I.
Duong, Timothy Q.
Lai, Hsin-Yi
Wang, Po-Hsun
Wu, Robby
Tsang, Siny
Chang, Jyh-Yeong
Li, Meng-Lin
Chen, You-Yin
Department of Electrical and Computer Engineering
Brain Research Center
Keywords: cerebral blood volume;forepaw electrical stimulation;hemodynamic response;hemoglobin oxygen saturation;total hemoglobin concentration;transcranial photoacoustic microscopy
Issue Date: 1-Jun-2012
Abstract: Optical imaging of changes in total hemoglobin concentration (HbT), cerebral blood volume (CBV), and hemoglobin oxygen saturation (SO2) provides a means to investigate brain hemodynamic regulation. However, high-resolution transcranial imaging remains challenging. In this study, we applied a novel functional photoacoustic microscopy technique to probe the responses of single cortical vessels to left forepaw electrical stimulation in mice with intact skulls. Functional changes in HbT, CBV, and SO2 in the superior sagittal sinus and different-sized arterioles from the anterior cerebral artery system were bilaterally imaged with unambiguous 36x65-mu m(2) spatial resolution. In addition, an early decrease of SO2 in single blood vessels during activation (i.e., 'the initial dip') was observed. Our results indicate that the initial dip occurred specifically in small arterioles of activated regions but not in large veins. This technique complements other existing imaging approaches for the investigation of the hemodynamic responses in single cerebral blood vessels. Journal of Cerebral Blood Flow & Metabolism (2012) 32, 938-951; doi:10.1038/jcbfm.2012.42; published online 4 April 2012
URI: http://hdl.handle.net/11536/16272
ISSN: 0271-678X
Volume: 32
Issue: 6
End Page: 938
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