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dc.contributor.authorChao, Jiun-Shuanen_US
dc.contributor.authorChao, Chun-Chiehen_US
dc.contributor.authorChang, Chu-Lien_US
dc.contributor.authorChiu, Yi-Rongen_US
dc.contributor.authorYuan, Chiun-Jyeen_US
dc.date.accessioned2014-12-08T15:23:06Z-
dc.date.available2014-12-08T15:23:06Z-
dc.date.issued2012-07-01en_US
dc.identifier.issn1073-6085en_US
dc.identifier.urihttp://hdl.handle.net/11536/16256-
dc.description.abstractSingle-vector Tet-on systems were developed to enable the tight regulation of transgenes in mammalian cells with a low dosage of doxycycline. Both the regulatory and the responsive units were integrated in a single vector and separated by a short DNA segment (214 bp). In the developed single-vector Tet-on systems, a high level of expression of the transgene can be induced by doxycycline at a concentration of as low as 1 ng/ml, which is 500-1,000 times lower than that usually utilized in other Tet-on systems. The single-vector Tet-on system developed here exhibited 3.5-10.8 times greater inducibility of the transgene in response to doxycycline than did a dual-vector system from a commercial source. Further studies indicate that the basal activity of Tet-on systems depends greatly on the strength of the promoter that controls the transactivator. The basal activity of Tet-on systems was high when the transactivator that was directed by the human cytomegalovirus promoter, and it was almost undetectable when the transactivator was placed under the control of a moderate strength mouse mammary tumor virus promoter. Moreover, the introduction of selectable markers allows the developed single-vector Tet-on systems to facilitate the generation of conditional transgenic cells and animals with high inducibility, low basal activity and detrimental effects of the long-term administration of doxycycline.en_US
dc.language.isoen_USen_US
dc.subjectTetracyclineen_US
dc.subjectTet-on inducible systemen_US
dc.subjectSingle-vectoren_US
dc.subjectTransgenic cellen_US
dc.subjectCMVen_US
dc.subjectMMTVen_US
dc.titleDevelopment of Single-Vector Tet-on Inducible Systems with High Sensitivity to Doxycyclineen_US
dc.typeArticleen_US
dc.identifier.journalMOLECULAR BIOTECHNOLOGYen_US
dc.citation.volume51en_US
dc.citation.issue3en_US
dc.citation.epage240en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.department分子醫學與生物工程研究所zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.contributor.departmentInstitute of Molecular Medicine and Bioengineeringen_US
dc.identifier.wosnumberWOS:000304404900005-
dc.citation.woscount2-
Appears in Collections:Articles


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