標題: Liposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch
作者: Chen, Chia-Hung
Lin, Yu-Ling
Liu, Yen-Ku
He, Pei-Juin
Lin, Ching-Min
Chiu, Yi-Han
Wu, Chang-Jer
Cheng, Tian-Lu
Liu, Shih-Jen
Liao, Kuang-Wen
生物科技學系
Department of Biological Science and Technology
關鍵字: liposome-PEG-PEI complex;adjuvant;class switch;immunomodulator;vaccine
公開日期: 2012
摘要: The aim of vaccination is to induce appropriate immunity against pathogens. Antibody-mediated immunity is critical for protection against many virus diseases, although it is becoming more evident that coordinated, multifunctional immune responses lead to the most effective defense. Specific antibody (Ab) isotypes are more efficient at protecting against pathogen invasion in different locations in the body. For example, compared to other Ab isotypes, immunoglobulin (Ig) A provides more protection at mucosal areas. In this study, we developed a cationic lipopolymer (liposome-polyethylene glycol-polyethyleneimine complex [LPPC])-adjuvant that strongly adsorbs antigens or immunomodulators onto its surface to enhance or switch immune responses. The results demonstrate that LPPC enhances uptake ability, surface marker expression, proinflammatory cytokine release, and antigen presentation in mouse-phagocytes. In contrast to Freund's adjuvant, LPPC preferentially activates-Th1-immunity against antigens in vivo. With lipopolysaccharides or CpG oligodeoxynucleotides, LPPC dramatically enhances the IgA or IgG2A proportion of total Ig, even in hosts that have developed Th2 immunities and high IgG1 serum titers. Taken together, the results demonstrate that the LPPC adjuvant not only increases the immunogenicity of antigens but also modulates host immunity to produce an appropriate Ab isotype by combining with immunomodulators.
URI: http://hdl.handle.net/11536/16195
ISSN: 1178-2013
期刊: INTERNATIONAL JOURNAL OF NANOMEDICINE
Volume: 7
結束頁: 607
顯示於類別:期刊論文


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