Title: Recovery Based Nanowire Field-Effect Transistor Detection of Pathogenic Avian Influenza DNA
Authors: Lin, Chih-Heng
Chu, Chia-Jung
Teng, Kang-Ning
Su, Yi-Jr
Chen, Chii-Dong
Tsai, Li-Chu
Yang, Yuh-Shyong
Department of Biological Science and Technology
Issue Date: 1-Feb-2012
Abstract: Fast and accurate diagnosis is critical in infectious disease surveillance and management. We proposed a DNA recovery system that can easily be adapted to DNA chip or DNA biosensor for fast identification and confirmation of target DNA. This method was based on the re-hybridization of DNA target with a recovery DNA to free the DNA probe. Functionalized silicon nanowire field-effect transistor (SiNW FET) was demonstrated to monitor such specific DNA-DNA interaction using high pathogenic strain virus hemagglutinin 1 (H1) DNA of avian influenza (AI) as target. Specific electric changes were observed in real-time for AI virus DNA sensing and device recovery when nanowire surface of SiNW FET was modified with complementary captured DNA probe. The recovery based SiNW FET biosensor can be further developed for fast identification and further confirmation of a variety of influenza virus strains and other infectious diseases. (C) 2012 The Japan Society of Applied Physics
URI: http://dx.doi.org/02BL02
ISSN: 0021-4922
DOI: 02BL02
Volume: 51
Issue: 2
End Page: 
Appears in Collections:Articles

Files in This Item:

  1. 000303481400095.pdf