標題: A T3 and T7 Recombinant Phage Acquires Efficient Adsorption and a Broader Host Range
作者: Lin, Tiao-Yin
Lo, Yi-Haw
Tseng, Pin-Wei
Chang, Shun-Fu
Lin, Yann-Tsyr
Chen, Ton-Seng
生物科技學系
Department of Biological Science and Technology
公開日期: 9-二月-2012
摘要: It is usually thought that bacteriophage T7 is female specific, while phage T3 can propagate on male and female Escherichia coli. We found that the growth patterns of phages T7M and T3 do not match the above characteristics, instead showing strain dependent male exclusion. Furthermore, a T3/7 hybrid phage exhibits a broader host range relative to that of T3, T7, as well as T7M, and is able to overcome the male exclusion. The T7M sequence closely resembles that of T3. T3/7 is essentially T3 based, but a DNA fragment containing part of the tail fiber gene 17 is replaced by the T7 sequence. T3 displays inferior adsorption to strains tested herein compared to T7. The T3 and T7 recombinant phage carries altered tail fibers and acquires better adsorption efficiency than T3. How phages T3 and T7 recombine was previously unclear. This study is the first to show that recombination can occur accurately within only 8 base-pair homology, where four-way junction structures are identified. Genomic recombination models based on endonuclease I cleavages at equivalent and nonequivalent sites followed by strand annealing are proposed. Retention of pseudo-palindromes can increase recombination frequency for reviving under stress.
URI: http://dx.doi.org/e30954
http://hdl.handle.net/11536/16139
ISSN: 1932-6203
DOI: e30954
期刊: PLOS ONE
Volume: 7
Issue: 2
結束頁: 
顯示於類別:期刊論文


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