Title: Enzyme-encapsulated silica nanoparticle for cancer chemotherapy
Authors: Chiu, Yi-Rong
Ho, Wei-Jen
Chao, Jiun-Shuan
Yuan, Chiun-Jye
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
Keywords: Enzyme encapsulation;Silicate nanoparticle;HRP;IAA;Prodrug cancer therapy;Nanomedicine
Issue Date: 1-Apr-2012
Abstract: A novel horseradish peroxidase-encapsulated silica nanoparticle (SNP) was generated in this study under relatively mild conditions. The generated enzyme-encapsulated SNP were relatively uniform in size (average 70 +/- 14.3 nm), monodispersed, and spherical, as characterized by transmission electron microscopy and scanning electron microscopy. The horseradish peroxidase encapsulated in silica nanoparticle exhibits biological properties, such as a pH-dependent activity profile and k(m) value, similar to that of free enzymes. Furthermore, enzyme-encapsulated SNP exhibited good operational stability for the repetitive usage with a relative standard deviation of 5.1 % ( n = 10) and a high stability for long term storage (>60 days) at 4 degrees C. The feasibility of using enzyme-encapsulated SNP in prodrug cancer therapy was also demonstrated by its capability to convert the prodrug indole-3-acetic acid into cytotoxic peroxyl radicals and trigger the death of tumor cells. These results indicate that the developed enzyme-encapsulated SNP has potential in the applications of prodrug cancer therapy.
URI: http://dx.doi.org/829
ISSN: 1388-0764
DOI: 829
Volume: 14
Issue: 4
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