標題: Enzyme-encapsulated silica nanoparticle for cancer chemotherapy
作者: Chiu, Yi-Rong
Ho, Wei-Jen
Chao, Jiun-Shuan
Yuan, Chiun-Jye
生物科技學系
分子醫學與生物工程研究所
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
關鍵字: Enzyme encapsulation;Silicate nanoparticle;HRP;IAA;Prodrug cancer therapy;Nanomedicine
公開日期: 1-四月-2012
摘要: A novel horseradish peroxidase-encapsulated silica nanoparticle (SNP) was generated in this study under relatively mild conditions. The generated enzyme-encapsulated SNP were relatively uniform in size (average 70 +/- 14.3 nm), monodispersed, and spherical, as characterized by transmission electron microscopy and scanning electron microscopy. The horseradish peroxidase encapsulated in silica nanoparticle exhibits biological properties, such as a pH-dependent activity profile and k(m) value, similar to that of free enzymes. Furthermore, enzyme-encapsulated SNP exhibited good operational stability for the repetitive usage with a relative standard deviation of 5.1 % ( n = 10) and a high stability for long term storage (>60 days) at 4 degrees C. The feasibility of using enzyme-encapsulated SNP in prodrug cancer therapy was also demonstrated by its capability to convert the prodrug indole-3-acetic acid into cytotoxic peroxyl radicals and trigger the death of tumor cells. These results indicate that the developed enzyme-encapsulated SNP has potential in the applications of prodrug cancer therapy.
URI: http://dx.doi.org/829
http://hdl.handle.net/11536/16096
ISSN: 1388-0764
DOI: 829
期刊: JOURNAL OF NANOPARTICLE RESEARCH
Volume: 14
Issue: 4
結束頁: 
顯示於類別:期刊論文


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