標題: Bostrycin, a novel coupling agent for protein immobilization and prevention of biomaterial-centered infection produced by Nigrospora sp No. 407
作者: Yang, Wen-Jen
Yang, Chih-Sheng
Huang, Chen-Ji
Chen, Ko-Shao
Lin, Shuen-Fuh
生物科技學系
Department of Biological Science and Technology
關鍵字: Bostrycin;Coupling agent;Surface modification;Protein immobilization;Bacteriostasis
公開日期: 10-May-2012
摘要: Bostrycin, a red antibacterial agent with tetrahydroanthraquinone structure, has been isolated from Nigrospora sp. No. 407. This study investigated the potential antibacterial and multifunctional properties of matrixes through immobilization of bostrycin on their surface for immobilization of protein and prevention of bacterial growth. Bostrycin was immobilized on nonwoven polypropylene (PP) fabric by a technique using glutaraldehyde and polyethyleneimine for the activation of the surface. Glucose oxidase immobilized on bostrycin-treated nonwoven PP fabric showed high activity. The immobilization process improved thermal stability of the enzymes. During repeated assay for 30 cycles, the enzyme activity dropped to only 70% of the initial activity. Both bostrycin-treated nonwoven PP fabric sample and subsequently immobilized glucose oxidase sample on the surface also still exhibited a bacteriostatic effect. This is the first study to show that bostrycin is a promising coupling agent for surface modification on matrix and its potential applications in protein immobilization and biomaterial-centered infection. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11536/15977
ISSN: 0141-0229
期刊: ENZYME AND MICROBIAL TECHNOLOGY
Volume: 50
Issue: 6-7
結束頁: 287
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