標題: Steric recognition of T-cell receptor contact residues is required to map mutant epitopes by immunoinformatical programmes
作者: Hsu, Shiou-Chih
Chang, Chih-Peng
Tsai, Chao-Yuan
Hsieh, Shih-Hung
Wu-Hsieh, Betty A.
Lo, Yu-Shu
Yang, Jinn-Moon
交大名義發表
National Chiao Tung University
關鍵字: CD8;cytotoxic T cells;major histocompatibility complexes;HLA;T-cell receptor;T cells;vaccines
公開日期: 1-六月-2012
摘要: MHC class I-restricted CD8 T-lymphocyte epitopes comprise anchor motifs, T-cell receptor (TCR) contact residues and the peptide backbone. Serial variant epitopes with substitution of amino acids at either anchor motifs or TCR contact residues have been synthesized for specific interferon-? responses to clarify the TCR recognition mechanism as well as to assess the epitope prediction capacity of immunoinformatical programmes. CD8 T lymphocytes recognise the steric configuration of functional groups at the TCR contact side chain with a parallel observation that peptide backbones of various epitopes adapt to the conserved conformation upon binding to the same MHC class I molecule. Variant epitopes with amino acid substitutions at the TCR contact site are not recognised by specific CD8 T lymphocytes without compromising their binding capacity to MHC class I molecules, which demonstrates two discrete antigen presentation events for the binding of peptides to MHC class I molecules and for TCR recognition. The predicted outcome of immunoinformatical programmes is not consistent with the results of epitope identification by laboratory experiments in the absence of information on the interaction with TCR contact residues. Immunoinformatical programmes based on the binding affinity to MHC class I molecules are not sufficient for the accurate prediction of CD8 T-lymphocyte epitopes. The predictive capacity is further improved to distinguish mutant epitopes from the non-mutated epitopes if the peptideTCR interface is integrated into the computing simulation programme.
URI: http://hdl.handle.net/11536/15966
ISSN: 0019-2805
期刊: IMMUNOLOGY
Volume: 136
Issue: 2
結束頁: 139
顯示於類別:期刊論文


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  1. 000303114300004.pdf