標題: Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis
作者: Lee, C. -N.
Wang, Y. -M.
Lai, W. -F.
Chen, T. -J.
Yu, M. -C.
Fang, C. -L.
Yu, F. -L.
Tsai, Y. -H.
Chang, W. H. -S.
Zuo, C. S.
Renshaw, P. F.
生物科技學系
Department of Biological Science and Technology
關鍵字: Diagnosis;extrapulmonary;magnetic resonance imaging;Mycobaterium tuberculosis;nanoparticle
公開日期: 1-六月-2012
摘要: Clin Microbiol Infect 2012; 18: E149E157 Abstract The limited sensitivity of serological tests for mycobacterial antigens has encouraged the development of a nanoparticle probe specific for the extrapulmonary form of Mycobacterium tuberculosis (Mtb). We developed an innovative probe comprised of super-paramagnetic iron oxide (SPIO) nanoparticles conjugated with Mtb surface antibody (MtbsAb-nanoparticles) to provide ultrasensitive imaging of biomarkers involved in extrapulmonary Mtb infection. MtbsAb-nanoparticles were significantly conjugated with Mtb bacilli. The extent of contrast enhancement reduction on magnetic resonance imaging (MRI) for Mtb and human monocytic THP1 cells was proportional to the concentration of MtbsAb-nanoparticles. When MtbsAb-nanoparticles were intravenously injected into mice bearing Mtb granulomas, the granulomatous site showed a 14-fold greater reduction in signal intensity enhancement on T2-weighted MR images compared with an opposing site that received PBS injection. Mtb sAb-nanoparticles represent a new non-invasive technology for the diagnosis of extrapulmonary Mtb.
URI: http://hdl.handle.net/11536/15953
ISSN: 1198-743X
期刊: CLINICAL MICROBIOLOGY AND INFECTION
Volume: 18
Issue: 6
結束頁: E149
顯示於類別:期刊論文


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