標題: Targeted RGD nanoparticles for highly sensitive in vivo integrin receptor imaging
作者: Lin, Ren-Yen
Dayananda, Kasala
Chen, Ting-Jung
Chen, Chiao-Yun
Liu, Gin-Chung
Lin, Kun-Liang
Wang, Yun-Ming
分子醫學與生物工程研究所
Institute of Molecular Medicine and Bioengineering
關鍵字: superparamagnetic nanoparticles (SPIO);magnetic resonance imaging (MRI);fluorescence;cell;peptide
公開日期: 1-一月-2012
摘要: A new magnetic resonance imaging (MRI) contrast bearing RGD peptide is reported. In this study, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles with various sizes were prepared. Particles sizes between 6 and 13?nm were tuned by varying the stirring rate. Remarkable negative contrast was observed because USPIO nanoparticles (13.1?+/-?2.1?nm) generated high transversal relaxivity r2 (188?+/-?3?m m-1?s-1) and saturation magnetization (94?emu?g-1 Fe). The USPIO nanoparticles were coated with PDA [2-(pyridyldithio)-ethylamine; PDA nanoparticles] containing functional polymer, which can be readily synthesized by Michael addition. The PDA nanoparticles were conjugated with RGD peptide (RGD nanoparticles) for targeting the specific site. The target specificity and high relaxivity allowed RGD nanoparticles to differentiate the expression level of integrin receptor on several cell lines and tumors (MCF-7, A-549, HT-29 and HT-1080) by in vitro and in vivo MR imaging. Importantly, a remarkable negative contrast (-51.3?+/-?6.7%) was observed for in vivo MR imaging of MCF-7 tumor. This result implies that the RGD nanoparticles that greatly enhance the MR imaging are highly sensitive for early stage tumor detection. Copyright (c) 2012 John Wiley & Sons, Ltd.
URI: http://dx.doi.org/10.1002/cmmi.457
http://hdl.handle.net/11536/15852
ISSN: 1555-4309
DOI: 10.1002/cmmi.457
期刊: CONTRAST MEDIA & MOLECULAR IMAGING
Volume: 7
Issue: 1
起始頁: 7
結束頁: 18
顯示於類別:期刊論文