標題: High Growth Rate Downregulates fumA mRNA Transcription but Is Dramatically Compensated by Its mRNA Stability in Escherichia coli
作者: Lin, Hsiao-Hsien
Lin, Ching-Hsueh
Hwang, Shiaw-Min
Tseng, Ching-Ping
生物科技學系
Department of Biological Science and Technology
公開日期: 1-五月-2012
摘要: Little is known about the association among the transcription, post-transcription, and protein production of the fumA gene. This study demonstrates that increasing growth rate (k) from 0.24/h to 0.96/h causes a marked eightfold reduction in fumA transcription as assessed using the beta-galactosidase activity from fumA promoter fused with a lacZ reporter. It was further confirmed using Northern blot analysis. Most interestingly, the FumA protein levels remained unchanged over the growth rate, as indicated by Western blot analysis. Therefore, whether the reduced fumA mRNA expression under the high growth rate can be overcome by increasing the stability of the fumA mRNA was tested. The half-life of fumA mRNA was established to significantly increase by fivefold when the growth rate was increased to 0.96/h. This finding suggests that the cells could turn down the expression of fumA mRNA because of increased stability of its mRNA under the high growth rate. This notion indicates that mRNA stability plays an essential role in maintaining a critical cellular level of a given protein when the mRNA transcript is downregulated by a metabolic event.
URI: http://dx.doi.org/10.1007/s00284-012-0087-6
http://hdl.handle.net/11536/15760
ISSN: 0343-8651
DOI: 10.1007/s00284-012-0087-6
期刊: CURRENT MICROBIOLOGY
Volume: 64
Issue: 5
起始頁: 412
結束頁: 417
顯示於類別:期刊論文


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  1. 000301841000002.pdf