標題: R432 is a key residue for the multiple functions of Ndt80p in Candida albicans
作者: Yang, Yun Liang
Wang, Chih Wei
Leaw, Shiang Ning
Chang, Te Pin
Wang, I. Chin
Chen, Chia Geun
Fan, Jen Chung
Tseng, Kuo Yun
Huang, Szu Hsuan
Chen, Chih Yu
Hsiao, Ting Yin
Hsiung, Chao Agnes
Chen, Chiung Tong
Hsiao, Chwan Deng
Lo, Hsiu Jung
生物科技學系
分子醫學與生物工程研究所
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
關鍵字: Candida albicans;Ndt80;Protein-DNA interaction;MSE;Binding assay
公開日期: 1-Mar-2012
摘要: Ndt80p is an important transcription modulator to various stress-response genes in Candida albicans, the most common human fungal pathogen in systemic infections. We found that Ndt80p directly regulated its target genes, such as YHB1, via the mid-sporulation element (MSE). Furthermore, the ndt80 (R432A) allele, with a reduced capability to bind MSE, failed to complement the defects caused by null mutations of NDT80. Thus, the R432 residue in the Ndt80p DNA-binding domain is involved in all tested functions, including cell separation, drug resistance, nitric oxide inactivation, germ tube formation, hyphal growth, and virulence. Hence, the importance of the R432 residue suggests a novel approach for designing new antifungal drugs by blocking the interaction between Ndt80p and its targets.
URI: http://dx.doi.org/10.1007/s00018-011-0849-5
http://hdl.handle.net/11536/15603
ISSN: 1420-682X
DOI: 10.1007/s00018-011-0849-5
期刊: CELLULAR AND MOLECULAR LIFE SCIENCES
Volume: 69
Issue: 6
起始頁: 1011
結束頁: 1023
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