標題: Histidine-containing Phosphotransfer Protein-B (HptB) Regulates Swarming Motility through Partner-switching System in Pseudomonas aeruginosa PAO1 Strain
作者: Bhuwan, Manish
Lee, Hui-Ju
Peng, Hwei-Ling
Chang, Hwan-You
生物科技學系
Department of Biological Science and Technology
公開日期: 13-一月-2012
摘要: The histidine-containing phosphotransfer protein-B (HptB; PA3345) is an intermediate protein involved in transferring a phosphoryl group from multiple sensor kinases to the response regulator PA3346 in Pseudomonas aeruginosa PAO1. The objective of this study was to elucidate the biological significance of the HptB-PA3346 interaction and the regulatory mechanisms thereafter. The transcription profiling analysis of an hptB knock-out mutant showed that the expression of a number of motility-related genes was altered consistent with the non-swarming phenotype observed for the mutant. Domain analysis indicated that the PA3346 C-terminal region (PA3346C) exhibits similar to 30% identity with the anti-sigma factor SpoIIAB of Bacillus subtilis. The presence of Ser/Thr protein kinase activity targeting an anti-sigma antagonist, PA3347, at Ser-56 was confirmed in PA3346C using an in vitro phosphorelay assay. Furthermore, PA3346C and the anti-sigma(28) factor FlgM were found to interact with PA3347 individually both in vivo and in vitro. FlgM displaced PA3346C in binding of PA3347 and was then competitively displaced by sigma(28) from the PA3347-FlgM complex, forming a phosphorylation-dependent partner-switching system. The significance of PA3347 phosphorylation in linking the partner-switching system and swarming motility was established by analyzing the swarming phenotype of the PA3347 knock-out mutant and its complement strains.
URI: http://dx.doi.org/10.1074/jbc.M111.256586
http://hdl.handle.net/11536/15259
ISSN: 0021-9258
DOI: 10.1074/jbc.M111.256586
期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
Volume: 287
Issue: 3
起始頁: 1903
結束頁: 1914
顯示於類別:期刊論文


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  1. 000299321000031.pdf