標題: Leucine pools in Escherichia coli biofilm discovered by Raman imaging
作者: Venkata, Hemanth Nag Noothalapati
Nomura, Nobuhiko
Shigeto, Shinsuke
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: Raman microspectroscopy;label-free imaging;biofilms;leucine
公開日期: 1-十一月-2011
摘要: In structured communities of bacteria known as biofilms, a variety of biomolecules have been shown to play a unique role as signals and/or regulators in biofilm formation. Here, we report that high levels of the amino acid leucine ( leucine pool) were detected, for the first time, within microcolonies in a 30-h-old Escherichia coli biofilm by Raman imaging. Localization of leucine revealed by multifrequency Raman images indicates leucine accumulation during the early stage of the E. coli biofilm formation, which may have resulted from physiological environment-specific metabolic adaptation. We demonstrate that our label-free Raman imaging method provides a useful platform for directly identifying still unknown natural products produced in biofilms as well as for visualizing heterogeneous distributions of biofilm constituents in situ. Copyright (C) 2011 John Wiley & Sons, Ltd.
URI: http://dx.doi.org/10.1002/jrs.2952
http://hdl.handle.net/11536/14940
ISSN: 0377-0486
DOI: 10.1002/jrs.2952
期刊: JOURNAL OF RAMAN SPECTROSCOPY
Volume: 42
Issue: 11
起始頁: 1913
結束頁: 1915
顯示於類別:期刊論文


文件中的檔案:

  1. 000297509800001.pdf